Devaki A. Kelkar, PhD.
Dr Kelkar's research training at CCMB, Hyderabad was in fluorescence and other spectroscopic techniques to study lipid protein interactions. As a post-doc, Dr. Kelkar switched gears to more complex proteins, initially studying molten globule states of pro-lactin and spectrin at CCMB and at OHSU, Portland worked on the co-translational folding of the RFP variant, m-cherry. On her return from USA, she worked at a private data curation firm in Pune as a Domain Expert. There she worked on designing and building databases for data curation of data relationships such as protein mutations and phenotypes, SNP-disease data, and microbiome data among others. In addition, she used R to create scripts to semi-automate the data curation process. Along with other members of the team, she developed a product to gather metadata for datasets in NCBI GEO for specific search words with filters and used text mining to classify GEO metadata as test/control cases. This work was presented as a poster at the 10th International Biocuration Conference. At Prashanti Cancer Care Mission she has built a data entry and management system to faciliated the creation of a database of breast cancer patients. She has created the database schema based on input from the clinicians at Prashanti and used that to create a systematic data entry portal with restricted vocabulary and partially in built data verification. The portal is written in python with a command line interface linked to a local SQLite database. This is in the process of being migrated to a server based mySQL database that can be linked to IHC and fluorescence imaging data at collaborative labs at IISER, Pune. She is also involved in building workflows for analysis of expression and mutation data from publicly available datasets such as TCGA and METABRIC and their correlation to clinico-pathological parameters.
Center for Translational Cancer Research
and Prashanti Cancer Care Mission
Flat No. 1-2, Kapilvastu, Senapati Bapat Road,
Phone: +91 (20) 69000710 / 25656110 / 25650269
Areas of expertise
Data Management and Analysis
1999 - 2005 PhD in Life Sciences, Jawaharlal Nehru University, New Delhi.
1997 - 1999 Masters in Science, Zoology Department, University of Pune, India.
1994 - 1997 Bachelors in Science Dyal Singh College, Kurukshetra University, Kurukshetra
2018 Consultant, Biology Department, IISER, Pune.
2014 - 2017 Domain Consultant, Athena Consulting, Pune.
2007 - 2012 Postdoctoral Fellow. Department of Biochemistry, Oregon Health and Science University, Portland, OR
2005 - 2006 Postdoctoral Fellow, Centre for Cellular and Molecular Biology, Hyderabad, India.
Fellowships and Awards
2005 IUBMB and SIGMA ALDRICH Corporation, Travel Scholarship Award
2003 - 2004 Senior Research Fellowship, CSIR India
2002 - 2003 Senior Research Fellowship, UGC, India
1999 - 2002 Junior Research Fellowship, UGC, India.
1999 Graduate Aptitude Test (GATE) Fellowship, (97.71 Percentile with an All India Rank of 31)
1997 Silver Jubilee Prize , University of Pune, Deparment of Zoology.
Kelkar, D.A., Khushoo, A., Yang, Z., Skach, W.R. (2012) Kinetic analysis of ribosome-bound fluorescent proteins reveals an early, stable, cotranslational folding intermediate. J. Biol. Chem. 287:2568-2578.
Kelkar, D.A., Chaudhuri, A., Haldar, S., Chattopadhyay, A. (2010) Exploring tryptophan dynamics in acid-induced molten globule state of bovine alpha-lactalbumin: a wavelength-selective fluorescence approach. Eur. Biophys. J. 39:1453-1463.
Chattopadhyay, A., Rawat, S.S., Greathouse, D.V., Kelkar, D.A., Koeppe R.E. (2008) Role of tryptophan residues in gramicidin channel organization and function. Biophys. J. 95:166-175
Kelkar, D.A., and Chattopadhyay, A. (2007) Modulation of gramicidin conformation and organization by hydrophobic mismatch in saturated phosphatidylcholine bilayers. Biochim. Biophys. Acta 1768:1103-1113.
Kelkar, D.A., and Chattopadhyay, A. (2007) The gramicidin ion channel: a model membrane protein. Biochim. Biophys. Acta 1768:2011-2025.
Chakrabarti, A., Kelkar, D.A., and Chattopadhyay, A. (2006) Spectrin organization and dynamics: new insights. Bioscience Rep. 26:369-386.
Kelkar, D.A., and Chattopadhyay, A. (2006) Membrane interfacial localization of aromatic amino acids and membrane protein function. J. Biosci. 31:297-302
Kelkar, D.A., and Chattopadhyay, A. (2006) Monitoring ion channel conformations in membranes utilizing a novel dual fluorescence quenching approach. Biochem. Biophys. Res. Commun. 343:483-488
Kelkar, D.A., Chattopadhyay, A., Chakrabarti, A., and Bhattacharyya, M. (2005) Effect of ionic strength on the organization and dynamics of tryptophan residues in spectrin: a fluorescence approach. Biopolymers 77:325-334.
Rawat, S.S., Kelkar, D.A., and Chattopadhyay, A. (2005) Effect of structural transition of the host assembly on dynamics of an ion channel peptide: a fluorescence approach. Biophys. J. 89:3049-3058.
Kelkar, D.A., and Chattopadhyay, A. (2005) Effect of graded hydration on the organization and dynamics of an ion channel: a fluorescence approach. Biophys. J. 88:1070-1080.
Chattopadhyay, A., Arora, A., and Kelkar, D.A. (2005) Dynamics of a membrane-bound tryptophan analog in environments of varying hydration: a fluorescence approach. Eur. Biophys J. 35:62-71
Raghuraman, H., Kelkar, D.A., and Chattopadhyay, A. (2005) Novel insights into protein structure and dynamics utilizing the red edge excitation shift approach. Reviews in Fluorescence 2005. Geddes C.D., and Lakowicz, J.R. Editors. Springer, New York. pp. 199-222.
Rawat, S.S.,* Kelkar, D.A.,* and Chattopadhyay, A. (2004) Monitoring gramicidin conformations in membranes: a fluorescence approach. Biophys. J. 87:831-843. (* Equal contribution)
Kelkar, D.A., and Chattopadhyay, A. (2004) Depth-dependent solvent relaxation in reverse
micelles: a fluorescence approach. J. Phys. Chem. B 108:12151-12158.
Raghuraman, H., Kelkar, D.A., and Chattopadhyay, A. (2003) Novel Insights into Membrane Protein Structure and Dynamics utilizing the Wavelength-Selective Fluorescence Approach. Proc. Ind. Natl. Sci. Acad. A 69:25-35.
Kelkar, D.A., Ghosh, A., and Chattopadhyay, A. (2003) Modulation of fluorophore environment in host membranes of varying charge. J. Fluoresc. 13:459-466.
Chattopadhyay, A., Rawat, S.S., Kelkar, D.A., Ray, S., and Chakrabarti, A. (2003) Organization and dynamics of tryptophan residues in erythroid spectrin: novel structural of denatured spectrin revealed by the wavelength-selective fluorescence approach. Protein Sci. 12:2389-2403.